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Effect of 9-TB on cortical immune infiltration and neuronal injury after CNS ischemia-reperfusion. (A) 9-TB limits cortical injury in 3VO/LPS exposed mice, measured by areas of attenuated <t>MAP2</t> staining. ****p < 0.0001, SHAM/SAL/VEH (n=5) vs. 3VO/LPS/VEH (n=6); ##p < 0.01, 3VO/LPS/VEH vs. 3VO/LPS/9-TB (n=7); SHAM/SAL/9-TB (n=6). (B-C) Effects of 3VO/LPS and 9-TB on the accumulation of EGFP(+) myeloid cells within the cerebral cortex of LysM-EGFP mice three days following ischemia-reperfusion. ***p < 0.001 SHAM/SAL vs. 3VO/LPS. Scale bar = 200 μm. (D) Association between cortical myeloid cell accumulation and MAP2(+) loss. Fine, curved lines around slope represent 95% confidence interval. Y = 145X - 957. (E) IHC staining of cortical sections for MAP2 depicting the relationship between EGFP(+) granulocyte accumulation with injured (left, MAP2Low outlined in white) or uninjured (right, MAP2High) parenchyma. Scale bar = 200 μm. Data are expressed as the mean ± S.D. (n = 5–7 mice/group). Statistical comparisons were made by 2-way ANOVA.
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Validation of hemagglutination inhibition (HI) testing protocol using clade 2.3.4.4 goose Guangdong lineage highly pathogenic H5 influenza A virus (GsGD-HP-H5 IAV) and North American lineage low pathogenic (LP) H5 influenza A virus (IAV) antigens tested against control antisera, sera from birds experimentally infected with GsGD-HP-H5 IAV and LP IAV, and field samples from wild birds taken prior to detection of clade 2.3.4.4 GsGD-HP-H5 IAV in North America. Twofold higher HI antibody titers to either clade 2.3.4.4 GsGD-HP-H5 IAV or North American lineage LP H5 IAV are in bold.
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Image Search Results


Effect of 9-TB on cortical immune infiltration and neuronal injury after CNS ischemia-reperfusion. (A) 9-TB limits cortical injury in 3VO/LPS exposed mice, measured by areas of attenuated MAP2 staining. ****p < 0.0001, SHAM/SAL/VEH (n=5) vs. 3VO/LPS/VEH (n=6); ##p < 0.01, 3VO/LPS/VEH vs. 3VO/LPS/9-TB (n=7); SHAM/SAL/9-TB (n=6). (B-C) Effects of 3VO/LPS and 9-TB on the accumulation of EGFP(+) myeloid cells within the cerebral cortex of LysM-EGFP mice three days following ischemia-reperfusion. ***p < 0.001 SHAM/SAL vs. 3VO/LPS. Scale bar = 200 μm. (D) Association between cortical myeloid cell accumulation and MAP2(+) loss. Fine, curved lines around slope represent 95% confidence interval. Y = 145X - 957. (E) IHC staining of cortical sections for MAP2 depicting the relationship between EGFP(+) granulocyte accumulation with injured (left, MAP2Low outlined in white) or uninjured (right, MAP2High) parenchyma. Scale bar = 200 μm. Data are expressed as the mean ± S.D. (n = 5–7 mice/group). Statistical comparisons were made by 2-way ANOVA.

Journal: Experimental and molecular pathology

Article Title: Effects of 9-t-butyl doxycycline on the innate immune response to CNS ischemia-reperfusion injury

doi: 10.1016/j.yexmp.2020.104601

Figure Lengend Snippet: Effect of 9-TB on cortical immune infiltration and neuronal injury after CNS ischemia-reperfusion. (A) 9-TB limits cortical injury in 3VO/LPS exposed mice, measured by areas of attenuated MAP2 staining. ****p < 0.0001, SHAM/SAL/VEH (n=5) vs. 3VO/LPS/VEH (n=6); ##p < 0.01, 3VO/LPS/VEH vs. 3VO/LPS/9-TB (n=7); SHAM/SAL/9-TB (n=6). (B-C) Effects of 3VO/LPS and 9-TB on the accumulation of EGFP(+) myeloid cells within the cerebral cortex of LysM-EGFP mice three days following ischemia-reperfusion. ***p < 0.001 SHAM/SAL vs. 3VO/LPS. Scale bar = 200 μm. (D) Association between cortical myeloid cell accumulation and MAP2(+) loss. Fine, curved lines around slope represent 95% confidence interval. Y = 145X - 957. (E) IHC staining of cortical sections for MAP2 depicting the relationship between EGFP(+) granulocyte accumulation with injured (left, MAP2Low outlined in white) or uninjured (right, MAP2High) parenchyma. Scale bar = 200 μm. Data are expressed as the mean ± S.D. (n = 5–7 mice/group). Statistical comparisons were made by 2-way ANOVA.

Article Snippet: Floating brain sections were washed with PBS and blocked in 10% goat serum for 1 hour at 20°C prior to immunohistochemical (IHC) staining with chicken polyclonal MAP2 antisera (EnCor, Gainesville, FL; 1:1000) incubated overnight at 4 °C followed by an Alexa Fluor TM 647 secondary antibody (Invitrogen, Carlsbad, CA; 1:500) for 1 hour at room temperature.

Techniques: Staining, Immunohistochemistry

Validation of hemagglutination inhibition (HI) testing protocol using clade 2.3.4.4 goose Guangdong lineage highly pathogenic H5 influenza A virus (GsGD-HP-H5 IAV) and North American lineage low pathogenic (LP) H5 influenza A virus (IAV) antigens tested against control antisera, sera from birds experimentally infected with GsGD-HP-H5 IAV and LP IAV, and field samples from wild birds taken prior to detection of clade 2.3.4.4 GsGD-HP-H5 IAV in North America. Twofold higher HI antibody titers to either clade 2.3.4.4 GsGD-HP-H5 IAV or North American lineage LP H5 IAV are in bold.

Journal: Journal of wildlife diseases

Article Title: LIMITED DETECTION OF ANTIBODIES TO CLADE 2.3.4.4 A/GOOSE/GUANGDONG/1/1996 LINEAGE HIGHLY PATHOGENIC H5 AVIAN INFLUENZA VIRUS IN NORTH AMERICAN WATERFOWL

doi:

Figure Lengend Snippet: Validation of hemagglutination inhibition (HI) testing protocol using clade 2.3.4.4 goose Guangdong lineage highly pathogenic H5 influenza A virus (GsGD-HP-H5 IAV) and North American lineage low pathogenic (LP) H5 influenza A virus (IAV) antigens tested against control antisera, sera from birds experimentally infected with GsGD-HP-H5 IAV and LP IAV, and field samples from wild birds taken prior to detection of clade 2.3.4.4 GsGD-HP-H5 IAV in North America. Twofold higher HI antibody titers to either clade 2.3.4.4 GsGD-HP-H5 IAV or North American lineage LP H5 IAV are in bold.

Article Snippet: North American H5 LP virus antigen included: LP-rgBWT/TX = reverse genetics-A/Blue-winged Teal/AI12-4150/Texas/2012 (ΔH5N2). b MN antigens included: LP-rgBWT/TX, HP-Chick/Neth = rg-A/Chicken/Netherland/EMC-3/2014 (ΔH5N8), and HP-SNOW/MO = rg-A/Snow Goose/Missouri/CC15-84a/2015 (ΔH5N2). c Wild bird samples with HI antibody titers twofold and onefold higher to clade 2.3.4.4 GsGD-HP-H5 IAV compared to North American lineage LP H5 IAV. d Wild bird samples that tested HI positive only for North American lineage LP H5 IAV. e Samples from Mallards experimentally infected with A/Mallard/Minnesota/355779/2000 (H5N2). f Control antisera (chicken) to A/Gyrfalcon/Washington/2014 (H5N8) (A/GYR/WA) provided by the National Veterinary Services Laboratories (NVSL), Veterinary Services, US Department of Agriculture.

Techniques: Biomarker Discovery, HI Assay, Virus, Control, Infection